Correlative Light and Electron MIcroscopy

Gebonden Engels 2012 9780124160262
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

Specificaties

ISBN13:9780124160262
Taal:Engels
Bindwijze:Gebonden

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Inhoudsopgave

<ol> <ol> <li>Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations</li> <li>Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy</li> <li>Correlative Fluorescence and Transmission Electron Microscopy in Tissues</li> <li>Correlative Light and Electron Microscopy in Parasite Research </li> <li>Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy</li> <li>3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection</li> <li>Correlative Light and Electron Microscopy of GFP</li> <li>Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging</li> <li>Correlated Light Microscopy and Electron Microscopy (CLEM): Search… and Find!</li> <li>Capturing Endocytic Segregation Events with HPF-CLEM</li> <li>Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms</li> <li>Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo</li> <li>Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers</li> <li>Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining</li> <li>Visualizing Proteins in Electron Micrographs at Nanometer Resolution</li> <li>Atmospheric Scanning Electron Microscope for Correlative Microscopy</li> <li>Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures</li> <li>Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms</li></ol></ol>

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        Correlative Light and Electron MIcroscopy